Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics

J. S. Song, K. Takimoto, M. Jeon, J. Vadakekalam, N. B. Ruparel, A. Diogenes

Research output: Research - peer-reviewArticle

  • 1 Citations

Abstract

Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alternative to conventional root canal treatment for immature teeth. These procedures rely on the transfer of apically positioned stem cells, including stem cells of the apical papilla (SCAP), into the root canal system. Although clinical success has been reported for these procedures, the predictability of expected outcomes and the organization of the newly formed tissues are affected by the lack of an available suitable scaffold that mimics the complexity of the dental pulp extracellular matrix (ECM). In this study, we evaluated 3 methods of decellularization of human dental pulp to be used as a potential autograft scaffold. Tooth slices of human healthy extracted third molars were decellularized by 3 different methods. One of the methods generated the maximum observed decellularization with minimal impact on the ECM composition and organization. Furthermore, recellularization of the scaffold supported the proliferation of SCAP throughout the scaffold with differentiation into odontoblast-like cells near the dentinal walls. Thus, this study reports that human dental pulp from healthy extracted teeth can be successfully decellularized, and the resulting scaffold supports the proliferation and differentiation of SCAP. The future application of this form of an autograft in REPs can fulfill a yet unmet need for a suitable scaffold, potentially improving clinical outcomes and ultimately promoting the survival and function of teeth with otherwise poor prognosis.

LanguageEnglish (US)
Pages640-646
Number of pages7
JournalJournal of Dental Research
Volume96
Issue number6
DOIs
StatePublished - Jun 1 2017

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Dental Pulp
Endodontics
Tooth
Stem Cells
Dental Pulp Cavity
Autografts
Extracellular Matrix
Odontoblasts
Third Molar
Organogenesis
Crowns
Mouth
Survival
Wounds and Injuries

Keywords

  • allografts
  • odontoblasts
  • regeneration
  • stem cells
  • tissue engineering
  • transplantation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Song, J. S., Takimoto, K., Jeon, M., Vadakekalam, J., Ruparel, N. B., & Diogenes, A. (2017). Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics. Journal of Dental Research, 96(6), 640-646. DOI: 10.1177/0022034517693606

Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics. / Song, J. S.; Takimoto, K.; Jeon, M.; Vadakekalam, J.; Ruparel, N. B.; Diogenes, A.

In: Journal of Dental Research, Vol. 96, No. 6, 01.06.2017, p. 640-646.

Research output: Research - peer-reviewArticle

Song, JS, Takimoto, K, Jeon, M, Vadakekalam, J, Ruparel, NB & Diogenes, A 2017, 'Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics' Journal of Dental Research, vol 96, no. 6, pp. 640-646. DOI: 10.1177/0022034517693606
Song JS, Takimoto K, Jeon M, Vadakekalam J, Ruparel NB, Diogenes A. Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics. Journal of Dental Research. 2017 Jun 1;96(6):640-646. Available from, DOI: 10.1177/0022034517693606
Song, J. S. ; Takimoto, K. ; Jeon, M. ; Vadakekalam, J. ; Ruparel, N. B. ; Diogenes, A./ Decellularized Human Dental Pulp as a Scaffold for Regenerative Endodontics. In: Journal of Dental Research. 2017 ; Vol. 96, No. 6. pp. 640-646
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