Rough titanium alloys regulate osteoblast production of angiogenic factors

Rene Olivares-Navarrete, Sharon L. Hyzy, Rolando A. Gittens I, Jennifer M. Schneider, David A. Haithcock, Peter F. Ullrich, Paul J. Slosar, Zvi Schwartz, Barbara D. Boyan

Research output: Contribution to journalArticle

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Abstract

Background context: Polyether-ether-ketone (PEEK) and titanium-aluminum- vanadium (titanium alloy) are used frequently in lumbar spine interbody fusion. Osteoblasts cultured on microstructured titanium generate an environment characterized by increased angiogenic factors and factors that inhibit osteoclast activity mediated by integrin α2β1 signaling. It is not known if this is also true of osteoblasts on titanium alloy or PEEK. Purpose: The purpose of this study was to determine if osteoblasts generate an environment that supports angiogenesis and reduces osteoclastic activity when grown on smooth titanium alloy, rough titanium alloy, or PEEK. Study design: This in vitro study compared angiogenic factor production and integrin gene expression of human osteoblast-like MG63 cells cultured on PEEK or titanium-aluminum-vanadium (titanium alloy). Methods: MG63 cells were grown on PEEK, smooth titanium alloy, or rough titanium alloy. Osteogenic microenvironment was characterized by secretion of osteoprotegerin and transforming growth factor beta-1 (TGF-β1), which inhibit osteoclast activity and angiogenic factors including vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), and angiopoietin-1 (ANG-1). Expression of integrins, transmembrane extracellular matrix recognition proteins, was measured by real-time polymerase chain reaction. Results: Culture on titanium alloy stimulated osteoprotegerin, TGF-β1, VEGF-A, FGF-2, and angiopoietin-1 production, and levels were greater on rough titanium alloy than on smooth titanium alloy. All factors measured were significantly lower on PEEK than on smooth or rough titanium alloy. Culture on titanium alloy stimulated expression of messenger RNA for integrins that recognize Type I collagen in comparison with PEEK. Conclusions: Rough titanium alloy stimulated cells to create an osteogenic-angiogenic microenvironment. The osteogenic-angiogenic responses to titanium alloy were greater than PEEK and greater on rough titanium alloy than on smooth titanium alloy. Surface features regulated expression of integrins important in collagen recognition. These factors may increase bone formation, enhance integration, and improve implant stability in interbody spinal fusions.

Original languageEnglish (US)
Pages (from-to)1563-1570
Number of pages8
JournalSpine Journal
Volume13
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Angiogenesis Inducing Agents
Titanium
Osteoblasts
Ketones
Ether
Integrins
Angiopoietin-1
Osteoprotegerin
Vanadium
Fibroblast Growth Factor 2
Osteoclasts
Aluminum
Vascular Endothelial Growth Factor A
Spinal Fusion
Extracellular Matrix Proteins
Collagen Type I
Osteogenesis
Transforming Growth Factor beta
Real-Time Polymerase Chain Reaction
Cultured Cells

Keywords

  • Angiogenesis
  • Osteoblast
  • PEEK
  • Polyether-ether-ketone
  • Titanium-aluminum-vanadium alloy

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Olivares-Navarrete, R., Hyzy, S. L., Gittens I, R. A., Schneider, J. M., Haithcock, D. A., Ullrich, P. F., ... Boyan, B. D. (2013). Rough titanium alloys regulate osteoblast production of angiogenic factors. Spine Journal, 13(11), 1563-1570. DOI: 10.1016/j.spinee.2013.03.047

Rough titanium alloys regulate osteoblast production of angiogenic factors. / Olivares-Navarrete, Rene; Hyzy, Sharon L.; Gittens I, Rolando A.; Schneider, Jennifer M.; Haithcock, David A.; Ullrich, Peter F.; Slosar, Paul J.; Schwartz, Zvi; Boyan, Barbara D.

In: Spine Journal, Vol. 13, No. 11, 11.2013, p. 1563-1570.

Research output: Contribution to journalArticle

Olivares-Navarrete, R, Hyzy, SL, Gittens I, RA, Schneider, JM, Haithcock, DA, Ullrich, PF, Slosar, PJ, Schwartz, Z & Boyan, BD 2013, 'Rough titanium alloys regulate osteoblast production of angiogenic factors' Spine Journal, vol 13, no. 11, pp. 1563-1570. DOI: 10.1016/j.spinee.2013.03.047
Olivares-Navarrete R, Hyzy SL, Gittens I RA, Schneider JM, Haithcock DA, Ullrich PF et al. Rough titanium alloys regulate osteoblast production of angiogenic factors. Spine Journal. 2013 Nov;13(11):1563-1570. Available from, DOI: 10.1016/j.spinee.2013.03.047

Olivares-Navarrete, Rene; Hyzy, Sharon L.; Gittens I, Rolando A.; Schneider, Jennifer M.; Haithcock, David A.; Ullrich, Peter F.; Slosar, Paul J.; Schwartz, Zvi; Boyan, Barbara D. / Rough titanium alloys regulate osteoblast production of angiogenic factors.

In: Spine Journal, Vol. 13, No. 11, 11.2013, p. 1563-1570.

Research output: Contribution to journalArticle

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abstract = "Background context: Polyether-ether-ketone (PEEK) and titanium-aluminum- vanadium (titanium alloy) are used frequently in lumbar spine interbody fusion. Osteoblasts cultured on microstructured titanium generate an environment characterized by increased angiogenic factors and factors that inhibit osteoclast activity mediated by integrin α2β1 signaling. It is not known if this is also true of osteoblasts on titanium alloy or PEEK. Purpose: The purpose of this study was to determine if osteoblasts generate an environment that supports angiogenesis and reduces osteoclastic activity when grown on smooth titanium alloy, rough titanium alloy, or PEEK. Study design: This in vitro study compared angiogenic factor production and integrin gene expression of human osteoblast-like MG63 cells cultured on PEEK or titanium-aluminum-vanadium (titanium alloy). Methods: MG63 cells were grown on PEEK, smooth titanium alloy, or rough titanium alloy. Osteogenic microenvironment was characterized by secretion of osteoprotegerin and transforming growth factor beta-1 (TGF-β1), which inhibit osteoclast activity and angiogenic factors including vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), and angiopoietin-1 (ANG-1). Expression of integrins, transmembrane extracellular matrix recognition proteins, was measured by real-time polymerase chain reaction. Results: Culture on titanium alloy stimulated osteoprotegerin, TGF-β1, VEGF-A, FGF-2, and angiopoietin-1 production, and levels were greater on rough titanium alloy than on smooth titanium alloy. All factors measured were significantly lower on PEEK than on smooth or rough titanium alloy. Culture on titanium alloy stimulated expression of messenger RNA for integrins that recognize Type I collagen in comparison with PEEK. Conclusions: Rough titanium alloy stimulated cells to create an osteogenic-angiogenic microenvironment. The osteogenic-angiogenic responses to titanium alloy were greater than PEEK and greater on rough titanium alloy than on smooth titanium alloy. Surface features regulated expression of integrins important in collagen recognition. These factors may increase bone formation, enhance integration, and improve implant stability in interbody spinal fusions.",
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AU - Hyzy,Sharon L.

AU - Gittens I,Rolando A.

AU - Schneider,Jennifer M.

AU - Haithcock,David A.

AU - Ullrich,Peter F.

AU - Slosar,Paul J.

AU - Schwartz,Zvi

AU - Boyan,Barbara D.

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N2 - Background context: Polyether-ether-ketone (PEEK) and titanium-aluminum- vanadium (titanium alloy) are used frequently in lumbar spine interbody fusion. Osteoblasts cultured on microstructured titanium generate an environment characterized by increased angiogenic factors and factors that inhibit osteoclast activity mediated by integrin α2β1 signaling. It is not known if this is also true of osteoblasts on titanium alloy or PEEK. Purpose: The purpose of this study was to determine if osteoblasts generate an environment that supports angiogenesis and reduces osteoclastic activity when grown on smooth titanium alloy, rough titanium alloy, or PEEK. Study design: This in vitro study compared angiogenic factor production and integrin gene expression of human osteoblast-like MG63 cells cultured on PEEK or titanium-aluminum-vanadium (titanium alloy). Methods: MG63 cells were grown on PEEK, smooth titanium alloy, or rough titanium alloy. Osteogenic microenvironment was characterized by secretion of osteoprotegerin and transforming growth factor beta-1 (TGF-β1), which inhibit osteoclast activity and angiogenic factors including vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), and angiopoietin-1 (ANG-1). Expression of integrins, transmembrane extracellular matrix recognition proteins, was measured by real-time polymerase chain reaction. Results: Culture on titanium alloy stimulated osteoprotegerin, TGF-β1, VEGF-A, FGF-2, and angiopoietin-1 production, and levels were greater on rough titanium alloy than on smooth titanium alloy. All factors measured were significantly lower on PEEK than on smooth or rough titanium alloy. Culture on titanium alloy stimulated expression of messenger RNA for integrins that recognize Type I collagen in comparison with PEEK. Conclusions: Rough titanium alloy stimulated cells to create an osteogenic-angiogenic microenvironment. The osteogenic-angiogenic responses to titanium alloy were greater than PEEK and greater on rough titanium alloy than on smooth titanium alloy. Surface features regulated expression of integrins important in collagen recognition. These factors may increase bone formation, enhance integration, and improve implant stability in interbody spinal fusions.

AB - Background context: Polyether-ether-ketone (PEEK) and titanium-aluminum- vanadium (titanium alloy) are used frequently in lumbar spine interbody fusion. Osteoblasts cultured on microstructured titanium generate an environment characterized by increased angiogenic factors and factors that inhibit osteoclast activity mediated by integrin α2β1 signaling. It is not known if this is also true of osteoblasts on titanium alloy or PEEK. Purpose: The purpose of this study was to determine if osteoblasts generate an environment that supports angiogenesis and reduces osteoclastic activity when grown on smooth titanium alloy, rough titanium alloy, or PEEK. Study design: This in vitro study compared angiogenic factor production and integrin gene expression of human osteoblast-like MG63 cells cultured on PEEK or titanium-aluminum-vanadium (titanium alloy). Methods: MG63 cells were grown on PEEK, smooth titanium alloy, or rough titanium alloy. Osteogenic microenvironment was characterized by secretion of osteoprotegerin and transforming growth factor beta-1 (TGF-β1), which inhibit osteoclast activity and angiogenic factors including vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), and angiopoietin-1 (ANG-1). Expression of integrins, transmembrane extracellular matrix recognition proteins, was measured by real-time polymerase chain reaction. Results: Culture on titanium alloy stimulated osteoprotegerin, TGF-β1, VEGF-A, FGF-2, and angiopoietin-1 production, and levels were greater on rough titanium alloy than on smooth titanium alloy. All factors measured were significantly lower on PEEK than on smooth or rough titanium alloy. Culture on titanium alloy stimulated expression of messenger RNA for integrins that recognize Type I collagen in comparison with PEEK. Conclusions: Rough titanium alloy stimulated cells to create an osteogenic-angiogenic microenvironment. The osteogenic-angiogenic responses to titanium alloy were greater than PEEK and greater on rough titanium alloy than on smooth titanium alloy. Surface features regulated expression of integrins important in collagen recognition. These factors may increase bone formation, enhance integration, and improve implant stability in interbody spinal fusions.

KW - Angiogenesis

KW - Osteoblast

KW - PEEK

KW - Polyether-ether-ketone

KW - Titanium-aluminum-vanadium alloy

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